BT210 Protocol 8 Menu
Objectives ||
Tools ||
Protocol ||
Results ||
Analysis ||
Quiz ||
QC ||
References
Back To BT210 Protocol List (1-16)
PROTOCOL 8 Objectives
To become familiar with Western blotting. This method involves transferring proteins in a gel to a membrane, then hybridizing a protein of interest to a labeled antibody probe. Following hybridization, the labeled probe is developed in order to determine its location, and by extension the location of the protein of interest. In this case, our protein of interest in Green Fluorescent Protein (GFP) isolated from pGLO-transformed E. coli cells. Our primary antibody is a rabbit polyclonal species specific to GFP, and our secondary antibody is an alkaline phosphatase labeled goat anti-rabbit species.
© Sonia Wallman, NHCTC. 1997