Protocols
PROPERTIES OF DNA
Protocol I:
ISOLATION AND SPOOLING OF DNA
Protocol II:
GENOME MAPPING USING RESTRICTION ENZYMES
Protocol III:
RFLP IN DNA FINGERPRINTING
Protocol IV:
FULL DNA FINGERPRINTING (RFLP+SOUTHERN)
Protocol V:
PCR IN DNA FINGERPRINTING
Protocol VI:
GENE CLONING
PROPERTIES OF PROTEINS
Protocol VII:
HYDROPHOBIC INTERACTION CHROMATOGRAPHY COUPLED WITH SDS-PAGE ELECTROPHORESIS
Protocol VIII:
PAGE/WESTERN FOR PROTEIN IDENTIFICATION
Protocol IX:
GENE EXPRESSION REGULATION
ADVENTURES WITH tPA
Protocol X:
ISOLATION OF mRNA
Protocol XI:
REVERSE TRANSCRIPTION OF mRNA INTO cDNA
Protocol XII:
PRODUCTION OF A cDNA LIBRARY
Protocol XIII:
COLONY SCREENING FOR tPA cDNA
Protocol XIV:
AMPLIFICATION OF COLONIES CONTAINING tPA cDNA
Protocol XV:
PLASMID MINI-PREPARATION
Protocol XVI:
SEQUENCING OF tPA cDNA
Instructions for Writing Lab Reports
©
Sonia Wallman, NHCTC. 1997
Protocols