BT 210

Lecture Outline- Week 14

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Technology and Discovery: Primers and Probes

I. Denaturation/Renaturation of DNA

A. Hybridization Defined

B. Theoretical Considerations (melting temperature)

-- variables affecting melting temperature

-- calculation of melting temperatures

II. Technical Applications of Hybridization

A. Primers

-- use in PCR

-- use in reverse transcription

B. Probes

-- use in Southern (and Northern) blotting

-- colony hybridization