Standard Operating Procedures
UPSTREAM PROCESSING
BATCH CULTURE OF
E. coli
RECOMBINANT FOR JELLYFISH GREEN FLUORESCENT PROTEIN
BATCH CULTURE OF RECOMBINANT tPA SECRETING CHO Cells (ATCC 9606)
PROCESS CONTROLLED FED-BATCH FERMENTATION OF RECOMBINANT HSA SECRETING
Pichia pastoris
DOWNSTREAM PROCESSING
ISOELECTRIC FOCUSING OF PROTEINS OF INTEREST: tPA, HSA, BSA, TRANSFERRIN, AND IgG
TANGENTIAL FLOW AND DIAFILTRATION OF HSA
ION EXCHANGE CHROMATOGRAPHY OF tPA
HSA PURIFICATION USING AFFINITY CHROMATOGRAPHY
QUALITY CONTROL TESTING
GRAM STAIN SOP
MYCOPLASMA TESTING SOP
LAL ASSAY - GEL CLOT SOP
TRYPAN BLUE ASSAY SOP
tPA ELISA SOP
tPA ACTIVITY ASSAY SOP
HSA ELISA SOP
SDS PAGE SOP
EQUIPMENT
APPLIKON BIOREACTOR SOP
BIO TEK MICROPLATE READER SOP
SANYO CO2 INCUBATOR SOP
LASER PARTICLE COUNTER SOP
BIOLOGIC LP SOP
SCOUT PRO BALANCE CALIBRATION SOP
SCOUT PRO BALANCE OPERATION SOP
DRUMMOND PIPETTE SOP
MINI STIRRERS SOP
GEL DOC SOP
BIOFLO 3000 SOP
SPECTROMETER SOP
EDT ph METER SOP
MARKET FORGE AUTOCLAVE SOP
SANYO AUTOCLAVE SOP
HIRAYAMA PORTABLE TOP LOADING AUTOCLAVE SOP
RCS PLUS AIR SAMPLER SOP
TOP
© 2000 Sonia Wallman, PhD
Standard
Operating Procedures